Duetta(ls)
Fluorescence and Absorbance Spectrometer
Duetta™ is a 3-in-1 spectrofluorometer that combines the functions of fluorescence and absorbance spectrometers simultaneously. Thanks to its high-speed, built-in CCD detector, the Duetta can acquire a full spectrum from 250 nm to 1,100 nm in less than one second, making it the fastest fluorescence spectrometer on the market.
Duetta uses A-TEEM™ technology to acquire absorbance, transmission, and fluorescence EEMs simultaneously – a true 3-in-1 instrument that corrects for the inner filter effect on the fly.
Data acquisition is more accurate because measurements are taken from the same spot without moving the sample and repeating the exercise.
To aid in the use of the Duetta and teach fluorescence spectroscopy principles in an undergraduate teaching laboratory setting, we have made a series of education labs. Designed to teach some of the most important concepts in fluorescence spectroscopy, these education labs can be used as is, or tailored to fit the needs of any undergraduate laboratory.
Why Choose Duetta(ls)
- Two in One!
- Fluorescence and Absorbance in one Spectrometer
- Full 3-D Fluorescence EEM Acquisition in less than one second
- High Fidelity Molecular Fingerprinting with unique A-TEEMTM
- EzSpecTM Touch-Screen Software
Key Advantages of Duetta(ls)
Fluorescence & Absorbance on one platform
Simultaneous measurement streamlines workflows
UV‑Vis‑NIR Detection
Covers broad spectral applications from UV through NIR
Primary & secondary inner filter effect compensation
Ensures accurate fluorescence quantification
Highest fluorescence sensitivity in its class
Detects ultra‑low concentration signals
Applications of Duetta(ls)
Protein Characterization
Detailed analysis of protein structure, folding, and conformational changes using fluorescence signatures.
DNA/RNA Analysis
Quantification and purity assessment of nucleic acids via absorbance and fluorescence measurements.
Fluorescence Labeling & Tag Quantification
Accurate determination of fluorescent probe incorporation and tag density in biomolecular assays.
Drug Interaction Studies
Evaluation of ligand–protein and drug–target binding affinities using spectroscopic titration curves.
Excipient & Formulation Analysis
Assessment of formulation stability and excipient compatibility by tracking spectral shifts and quenching effects.
Technical Specifications of Duetta(ls)
| Acquisition Modes | Absorbance and Fluorescence Spectrometer |
|---|---|
| Fluorescence Sensitivity | Water Raman SNR >6,000:1 RMS, 350 nm excitation, 5 nm slits |
| Spectral Acquisition Rate | 510,000 nm/min |
| EEM Acquisition Rate | As fast as 1 second (sample and wavelength dependent) |
| A-TEEM Acquisition Rate | As fast as 30 seconds (sample and wavelength dependent) |
| Fluorescence Detector | CCD/Spectrograph |
| Fluorescence Detector Range | 250 to 1,100 nm |
| Fluorescence Bandwidth | 1, 2, 3, 5,10, 20 nm (excitation and emission) |
| Light Source | 75 W Xenon arc lamp. Dedicated cartidge for snap-in replacement |
| Excitation/Absorbance Wavelength Range | 250 to 1,000 nm |
| Absorbance Detector | Silicon Photodiode |
| Absorbance Detector Range | 250 to 1,000 nm |
| Absorbance Bandwidth | 1, 2, 3, 5,10, 20 nm |
| Absorbance Range | 0 to 2 A |
| Absorbance Accuracy | +/- 0.02 A |
| Wavelength Accuracy | +/- 1 nm |
| Computer Requirements | Windows 10 or 11 64-bit OS, laptop or PC with at least 1 USB port (more for additional accessories) |
| Software | EzSpec™ |
| Dimensions (W x D x H) | 17 x 20.4 x 14.4 inches; 43.18 x 51.816 x 36.576 cm |
| Weight | 45 pounds; 20.4 kg |
| Polarizers (Time of Purchase) | Not Available |
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